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Yocon is committed to produce high-quality and brand serum-free cell culture products, laying a solid foundation for cell culture media solutions.

CIK Cells Serum-free Medium

This product is a serum-free culture kit for pure factor culture of CIK cells, suitable for CIK cells culture of peripheral blood and umbilical cord blood samples

Product use

YOCON serum-free culture kit for pure factor culture of CIK cells is suitable for CIK cell culture of peripheral blood and umbilical cord blood samples. After 14-16 days of culture, the cell count reaches at least 5.0 billion, and the positive rate is over 20%. This product is composed of a basic medium and an induced expansion kit, to provide an extremely easy-to-use solution for CIK cell culture.
Product video

Cytokine-induced killer (CIK) cell therapy is a type of adoptive immunotherapy that due to its high proliferation rate and anti-tumor characteristics, is being investigated to treat various solid tumors. Since advanced colorectal cancer (CRC) has high mortality and poor survival rates, and the efficacy of chemotherapy and radiotherapy is limited in treatment, the application of CIK cell therapy in CRC has been evaluated in numerous studies. This review aims to summarize the clinical studies that investigated the safety and clinical efficacy of CIK cell therapy in CRC. Therefore, 1,969 enrolled CRC patients in the clinical trials, of which 842 patients received CIK cells in combination with chemotherapy with or without dendritic cell (DC) infusions, were included in the present review. Furthermore, the signaling pathways involved in CIK cell therapy and novel methods for improving migration abilities are discussed.

The total number of 4-6 billion cells can be obtained after 14 days of culture in a 2 L system, and the proportion of CD3+CD56+ is stable at 10% to 20%.

Pure factor induction, with no trophoblast cells, with the simple and easy culturing method, when combined with the newly upgraded basic culture medium of immune cells, significantly improved the continuous expansion ability of cells after 7 consecutive days of culturing, and the continuous support ability of cells expanded from 2 L system to 3 L or even 4 L system after 14 days.

The basic culture medium has been filed with FDA DMF with the filing No. of 37874


Applicable samples

Product specification

Item No.

Brief description of performance

Peripheral blood

2 bottles of serum-free medium for CIK cells (1 L/bottle) + CIK cell induced expansion kits (peripheral blood)

NC0101+AN0101.2

The cell count reaches 5.0 billion or above, and the positive rate is 20% or above


Umbilical cord blood

2 bottles of serum-free medium for CIK cells (1 L/bottle) + CIK cell induced expansion kits (umbilical cord blood)

NC0101+AN0104.2


Product advantages

Standard operation for different samples

Provided that the initial PBMC inoculation conditions are controlled, the subsequent cultures can be carried out according to the recommended fluid replacement process

Stable culture results

This product has been on the market for 7 years, with highly reliable stability

Data download

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